shrna (shlent) or a scrambled control (shctrl) Search Results


lent shrna shlent  (Addgene inc)
96
Addgene inc lent shrna shlent
A. Volcano blot showing RNAs enriched or depleted in the DHX36 IP vs control IgG IP. P-values were derived using the Wald test and adjusted using Benjamini-Hochberg FDR correction. B. Gene ontology analysis by EnrichR software of the 500 most enriched RNAs in the DHX36 IP. C. Volcano blot showing RNAs enriched or depleted in the DHX36 IP upon <t>shRNA-mediated</t> <t>LENT</t> silencing. P-values were derived using the Wald test. D. KEGG pathways by EnrichR of RNAs enriched or depleted in DHX36 IP upon LENT depletion.
Lent Shrna Shlent, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+(shlent)+or+a+scrambled+control+(shctrl)/shRNA+(Plasmid+%2355783)/bio_rxiv__2025__02__03__636228-282-0-17
Average 96 stars, based on 1 article reviews
lent shrna shlent - by Bioz Stars, 2026-09
96/100 stars
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scramble control vector  (OriGene)
93
OriGene scramble control vector
A. Volcano blot showing RNAs enriched or depleted in the DHX36 IP vs control IgG IP. P-values were derived using the Wald test and adjusted using Benjamini-Hochberg FDR correction. B. Gene ontology analysis by EnrichR software of the 500 most enriched RNAs in the DHX36 IP. C. Volcano blot showing RNAs enriched or depleted in the DHX36 IP upon <t>shRNA-mediated</t> <t>LENT</t> silencing. P-values were derived using the Wald test. D. KEGG pathways by EnrichR of RNAs enriched or depleted in DHX36 IP upon LENT depletion.
Scramble Control Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+(shlent)+or+a+scrambled+control+(shctrl)/Scrambled+shRNA+control+in+pRFP-C-shLenti+vector/pmc06437924-93-29-34
Average 93 stars, based on 1 article reviews
scramble control vector - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
lt3gepir  (Addgene inc)
95
Addgene inc lt3gepir
A. Volcano blot showing RNAs enriched or depleted in the DHX36 IP vs control IgG IP. P-values were derived using the Wald test and adjusted using Benjamini-Hochberg FDR correction. B. Gene ontology analysis by EnrichR software of the 500 most enriched RNAs in the DHX36 IP. C. Volcano blot showing RNAs enriched or depleted in the DHX36 IP upon <t>shRNA-mediated</t> <t>LENT</t> silencing. P-values were derived using the Wald test. D. KEGG pathways by EnrichR of RNAs enriched or depleted in DHX36 IP upon LENT depletion.
Lt3gepir, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+(shlent)+or+a+scrambled+control+(shctrl)/LT3GEPIR+(Plasmid+%23111177)/pm41419583-81-11-17
Average 95 stars, based on 1 article reviews
lt3gepir - by Bioz Stars, 2026-09
95/100 stars
  Buy from Supplier

Image Search Results


A. Volcano blot showing RNAs enriched or depleted in the DHX36 IP vs control IgG IP. P-values were derived using the Wald test and adjusted using Benjamini-Hochberg FDR correction. B. Gene ontology analysis by EnrichR software of the 500 most enriched RNAs in the DHX36 IP. C. Volcano blot showing RNAs enriched or depleted in the DHX36 IP upon shRNA-mediated LENT silencing. P-values were derived using the Wald test. D. KEGG pathways by EnrichR of RNAs enriched or depleted in DHX36 IP upon LENT depletion.

Journal: bioRxiv

Article Title: Interaction of lncRNA LENT with DHX36 regulates translation and suppresses autophagy in melanoma

doi: 10.1101/2025.02.03.636228

Figure Lengend Snippet: A. Volcano blot showing RNAs enriched or depleted in the DHX36 IP vs control IgG IP. P-values were derived using the Wald test and adjusted using Benjamini-Hochberg FDR correction. B. Gene ontology analysis by EnrichR software of the 500 most enriched RNAs in the DHX36 IP. C. Volcano blot showing RNAs enriched or depleted in the DHX36 IP upon shRNA-mediated LENT silencing. P-values were derived using the Wald test. D. KEGG pathways by EnrichR of RNAs enriched or depleted in DHX36 IP upon LENT depletion.

Article Snippet: LENT shRNA (shLENT) or a scrambled control (shCTRL) were cloned in LT3GEPIR (a gift from Johannes Zuber; Addgene plasmid #111177; http://n2t.net/addgene:111177 ; RRID: Addgene_111177).

Techniques: Control, Derivative Assay, Software, shRNA

A. Bio-analyser analyses of precipitated RNA show strong enrichment of 18S and 28S rRNAs in the DHX36 IP but not the control IgG IP. B. Sucrose gradient separation of ribosomes with rRNAs measured by UV-light spectrometry. C. Immunoblots for DHX36, the initiation factor EIF4A2 and the large ribosomal subunit RPL36 after polysome separation. EIF4A2 is enriched in the 40S and RPL36 in the 80S and heavier polysome fractions. D. Presence of LENT or GAPDH mRNA in fractionated ribosomes as measured by RT-qPCR. E. Representative sucrose gradient separation of ribosomes with rRNAs measured by UV-light spectrometry. Fractions pooled for the 80S, LP and HP are indicated. F . Volcano blot showing RNAs enriched or depleted in the LP fraction from control or shLENT cells. G. Gene ontology analysis by EnrichR software of the 383 depleted RNAs in the shLENT LP fraction.

Journal: bioRxiv

Article Title: Interaction of lncRNA LENT with DHX36 regulates translation and suppresses autophagy in melanoma

doi: 10.1101/2025.02.03.636228

Figure Lengend Snippet: A. Bio-analyser analyses of precipitated RNA show strong enrichment of 18S and 28S rRNAs in the DHX36 IP but not the control IgG IP. B. Sucrose gradient separation of ribosomes with rRNAs measured by UV-light spectrometry. C. Immunoblots for DHX36, the initiation factor EIF4A2 and the large ribosomal subunit RPL36 after polysome separation. EIF4A2 is enriched in the 40S and RPL36 in the 80S and heavier polysome fractions. D. Presence of LENT or GAPDH mRNA in fractionated ribosomes as measured by RT-qPCR. E. Representative sucrose gradient separation of ribosomes with rRNAs measured by UV-light spectrometry. Fractions pooled for the 80S, LP and HP are indicated. F . Volcano blot showing RNAs enriched or depleted in the LP fraction from control or shLENT cells. G. Gene ontology analysis by EnrichR software of the 383 depleted RNAs in the shLENT LP fraction.

Article Snippet: LENT shRNA (shLENT) or a scrambled control (shCTRL) were cloned in LT3GEPIR (a gift from Johannes Zuber; Addgene plasmid #111177; http://n2t.net/addgene:111177 ; RRID: Addgene_111177).

Techniques: Control, Western Blot, Quantitative RT-PCR, Software

A-C. RNA-seq data showing the representation of LENT in the 80S, LP and HP fractions. The normalized number of reads are shown along with the adjusted p-value between the control and shLENT conditions. D . Venn diagrams comparing RNAs modulated in the DHX36 RIP in presence or absence of LENT silencing with those differentially present in the 80S and LP fractions. Significant Representation Factors are shown. E-F . RNA-seq data showing the representation of the indicated RNAs in the 80S, LP and HP fractions. The normalized number of reads are shown along with the adjusted p-value between the control and shLENT conditions. G. Immunoblots of the indicated proteins 48 hours following ASO-mediated LENT silencing in 501Mel cells. H. Immunoblots of the indicated proteins 48 hours following ASO-mediated LENT silencing in the IGR37 cell line or extracts from IGR37 CDX tumours from control or LENT ASO-injected mice. I. Expression of the indicated RNAs in ASO control or ASO LENT transfected cells represented as fold-change in RNA-seq (upper panel) or RT-qPCR (lower panel).

Journal: bioRxiv

Article Title: Interaction of lncRNA LENT with DHX36 regulates translation and suppresses autophagy in melanoma

doi: 10.1101/2025.02.03.636228

Figure Lengend Snippet: A-C. RNA-seq data showing the representation of LENT in the 80S, LP and HP fractions. The normalized number of reads are shown along with the adjusted p-value between the control and shLENT conditions. D . Venn diagrams comparing RNAs modulated in the DHX36 RIP in presence or absence of LENT silencing with those differentially present in the 80S and LP fractions. Significant Representation Factors are shown. E-F . RNA-seq data showing the representation of the indicated RNAs in the 80S, LP and HP fractions. The normalized number of reads are shown along with the adjusted p-value between the control and shLENT conditions. G. Immunoblots of the indicated proteins 48 hours following ASO-mediated LENT silencing in 501Mel cells. H. Immunoblots of the indicated proteins 48 hours following ASO-mediated LENT silencing in the IGR37 cell line or extracts from IGR37 CDX tumours from control or LENT ASO-injected mice. I. Expression of the indicated RNAs in ASO control or ASO LENT transfected cells represented as fold-change in RNA-seq (upper panel) or RT-qPCR (lower panel).

Article Snippet: LENT shRNA (shLENT) or a scrambled control (shCTRL) were cloned in LT3GEPIR (a gift from Johannes Zuber; Addgene plasmid #111177; http://n2t.net/addgene:111177 ; RRID: Addgene_111177).

Techniques: RNA Sequencing, Control, Western Blot, Injection, Expressing, Transfection, Quantitative RT-PCR

A. Immunoblots detecting the indicated proteins in control or LENT depleted cells. Panels show different exposures of the same immuoblot. B. Immunoblots detecting mitochondrial electron transport chain proteins in extracts from IGR-37 CDX tumours. C. RNA-seq data showing the representation of the indicated RNAs in the 80S, LP and HP fractions. The normalized number of reads are shown along with the adjusted p-value between the control and shLENT conditions. D. OxPhos proteins were detected by confocal microscopy immunofluorescence in control or LENT-silenced cells using phalloidin to stain the cytoplasm. Proteins were quantified in the nucleus using the DAPI signal and comparisons were made by Mann-Whitney test. Scale bars = 10 µM. Representative nuclei displaying strong OxPhos protein staining are indicated by arrows. E. Immunoblot of ATP5A1 in cytosolic or mitochondrial fractions upon LENT depletion. Ponceau is shown as loading control. *, P < 0.033; **, P < 0.0021; ***, P < 0.0002; ****, P < 0.0001.

Journal: bioRxiv

Article Title: Interaction of lncRNA LENT with DHX36 regulates translation and suppresses autophagy in melanoma

doi: 10.1101/2025.02.03.636228

Figure Lengend Snippet: A. Immunoblots detecting the indicated proteins in control or LENT depleted cells. Panels show different exposures of the same immuoblot. B. Immunoblots detecting mitochondrial electron transport chain proteins in extracts from IGR-37 CDX tumours. C. RNA-seq data showing the representation of the indicated RNAs in the 80S, LP and HP fractions. The normalized number of reads are shown along with the adjusted p-value between the control and shLENT conditions. D. OxPhos proteins were detected by confocal microscopy immunofluorescence in control or LENT-silenced cells using phalloidin to stain the cytoplasm. Proteins were quantified in the nucleus using the DAPI signal and comparisons were made by Mann-Whitney test. Scale bars = 10 µM. Representative nuclei displaying strong OxPhos protein staining are indicated by arrows. E. Immunoblot of ATP5A1 in cytosolic or mitochondrial fractions upon LENT depletion. Ponceau is shown as loading control. *, P < 0.033; **, P < 0.0021; ***, P < 0.0002; ****, P < 0.0001.

Article Snippet: LENT shRNA (shLENT) or a scrambled control (shCTRL) were cloned in LT3GEPIR (a gift from Johannes Zuber; Addgene plasmid #111177; http://n2t.net/addgene:111177 ; RRID: Addgene_111177).

Techniques: Western Blot, Control, RNA Sequencing, Confocal Microscopy, Immunofluorescence, Staining, MANN-WHITNEY